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Reading an HPLC chromatogram
Reversed-phase HPLC separates peptides by hydrophobicity. The main peak is your product; everything else is either an impurity or an artefact of…
Analytics
The last two percent is the expensive part, because the impurities that remain are structurally similar to the product. Going from 95 % to 98 % may double the purification…
2 min read · updated 12 11 月, 2026
The last two percent is the expensive part, because the impurities that remain are structurally similar to the product. Going from 95 % to 98 % may double the purification burden; 98 % to 99 % can double it again.
Before specifying 99 %, check three things: whether the assay your customer uses can distinguish it, whether the impurity at that level matters for the application, and whether your specification names the method. “≥ 98.0 % by RP-HPLC, C18, 214 nm, threshold 0.1 %” is a specification. “≥ 98 %” is an invitation to argue.
Also separate purity by area from peptide content by mass. A 98 % pure acetate salt can be 80 % peptide by weight once water and counter-ion are counted. If you buy by mass, you are buying the salt.
Need the specification, not the theory?
Tell us the sequence, purity target and volume — the reply includes the documents that will ship with the lot.